Catalogue | DB_001

Porcine induced pluripotent stem cells

This porcine iPSC line was reprogrammed from intramuscular fibroblasts and the cells available are banked at P20+. They have been tested for sterility, mycoplasma, expression of pluripotency markers and differentiation to ectoderm, mesoderm and endoderm by embryoid body differentiation. Upon revival, the cells can be differentiated using our validated protocols to adipocytes and multinucleated muscle cells. piPSCs provide a source of consistent, reliable cells for both R&D and production. A line adapted to suspension culture conditions is available upon request. Ask us about our enhanced data packs.

Porcine induced pluripotent stem cells

Product Features

Versitile

Versitile

Lines adapted to anchorage-dependent (plates, flasks and micro-carriers) and suspension-adapted (aggregate) culture systems

Pluripotent

Pluripotent

Able to differentiate into almost any cell type, including adipocytes, myocytes and myofibers.

Streamlined

Streamlined

Single starting cell for the creation of species-specific cell line models and product development

Porcine induced pluripotent stem cells
Dragon Biotechnologies
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This piPSC line was derived by the reprogramming of intramuscular fibroblasts using a non-integrative method to deliver human isoform c-Myc, Klf4, Oct4 and Sox2. piPSCs are adapted to feeder free culture on commercially available basement membrane extract.

Cell characterization

Pluripotency marker expression for piPSCs. Top) immunofluorescent staining for OCT4 and NANOG. Bottom) flow cytometry for OCT4, SOX2 and SSEA4

Suspension adaption

Adaption of iPSCs to aggregates/spheroids in suspension culture. Top) representative microscopy images of piPSC aggregates in suspension at Day 1 (left) and Day 3 (right) of suspension culture in a 250mL flask on an orbital shaker. Bottom) gene expression of pluripotency markers in suspension adapted piPSCs.

Differentiation characterization

Differentiation of piPSCs. Top) Embryoid bodies formed from piPSCs positive for the expression of the germ layer markers PAX6 (ectoderm), T (mesoderm) and SOX17 (endoderm). Middle) piPSCs differentiated to adipocytes were positive for Oil Red O staining and the marker genes PPARG and CEBPB with diminished expression of OCT4. Bottom) piPSCs differentiated to skeletal muscle were positive for multi nucleated fibres and the marker genes MyoD1 and MYH2 with diminished expression of OCT4.

Compatible Processes

Cell Growth Formats
Adherent
Suspension Adapted
Cell Culture Vessels
Well Plates
Flasks
Adherent Scale Up
Scale Down Suspension Systems

Product information

24-48 hours
Avg. Population Doubling Time
Adipogenic differentiation
0 days
28 days
Immortalisation Method
Induced pluripotency via non integrative reprogramming method.
Species (Latin)
Sus scrofa domesticus
Founder Animal
Male Duroc white
Working Cell Bank Passage Number
P20+
Maximum Passage Number Achieved
P60+
Quality Control
Sterility and mycoplasma testing
Format

Cryopreserved cells, one vial containing appx. 2 x 106 cells

User Storage
-150°C or LN2
Shipping Method
Dry ice for immediate ultra-cold storage